Protein-Lipid Interactions: Perspectives, Techniques and Challenges analyzes and covers a broad spectrum of protein-lipid interactions and presents new information in this area of research. The topics analyzed include: an update on the cholesterol effects of the nicotinic acetylcholine receptor; the modulation of lipid compositions for protein–lipid interactions, membrane dynamics and their structures; defining the moderate affinity of specific lipid binding sites on amphitropic enzymes; the lipid-dependent gating of ion channels; the use of novel imaging approaches to study phosphoinositide signaling in vesicle trafficking; the study of protein-lipid interplay in vital biological functions; and particularly important instances of protein–lipid interactions from 1900 to 2017.