"Over the last 35 years the term genetic engineering has been commonly used not only in science but also in others parts of society. Nowadays this name is often associated by the media forensic techniques to solve crimes, paternity, medical diagnosis and, gene mapping and sequencing. Currently, genetic engineering is widely used at various branches of medicine to produce vaccines, monoclonal antibodies, animals that can be used as models for diseases or to be used as organ donors. Another function of genetic engineering is gene therapy which aims to restore correct gene expression in cells that have a defective form. The popularization of genetic engineering is consequence of its wide use in laboratories around the world and, developing of modern and efficient techniques. The genetic engineering, often used with trivia, involves sophisticated techniques of gene manipulation, cloning and modification. Transformation protocols and retrieval of recombinants are essential to the success of genetic engineering. Genetic Engineering: Principles And Methods sheds light on new transformation strategies which can be used to increase the transformation efficiency in most species; topics ranging from the genetic engineering of microorganisms to produce antibiotics, the gene targeting and transformation in plants, the generation of marker-free plants in response to biosafety concerns, as well as the generation of transgenic animals and those derived through cloning are covered.. Genetic engineering offers potentially practical solution to look for alternatives. The book portrays on aspects of genome editing which will enable researchers to produce transgenic animals and plants in a more convenient and safer way to genetic modification of stem cells. Covering a wide range of topics in genetic engineering of microorganisms, plants and animals; this book will appeal to students, practitioners, as well as researchers. "